At Illumina high-throughput sequencing platform Library Construction Fast DNA Library Plus Prep Kit K004-A/K004-B
【Product Name】
Fast DNA Library Plus Prep Kit
【Cat. No./Spec.】
K004-A/24 rxns; K004-B/96 rxns; sample sack/ 6 rxns
【Product Description】
Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines fragmentation, end repair and end “A” tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. After fragmentation and end repair, the product can be directly ligated with adapter without additional purification, and the subsequent procedure is the same as that of #K001 Fast DNA Library Prep Kit. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.
【Sample Type】
Table 1 Recommended Inputs for Common DNA
| Application | Sample Type | Recommended Amount |
| Whole genome sequencing | High quality complex genomes | 50ng-1μg |
| Target capture sequencing of whole exome | High quality complex genomes | 10ng-1μg |
| Target capture sequencing of whole genome | FFPE DNA | ≥50ng |
| Whole genome sequencing | Microbial genome | 1ng-1μg |
| Whole genome sequencing(PCR-free) | High quality DNA | ≥50ng (no size selection) ≥200ng (size selection) |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.
【Components】
| Component | 24 rxns | 96 rxns |
| FEP Buffer | 120 μl | 480 μl |
| FEP Enzyme Mix | 240 μl | 2×480 μl |
| Fast DNA Ligase | 120 μl | 2×240 μl |
| Fast Ligation Buffer | 600 μl | 4×600 μl |
| 2× HIFI Library PCR Master Mix | 600 μl | 4×600 μl |
| Primer Mix* | 120 μl | 480 μl |
| Neutralization Buffer | 120 μl | 480 μl |
*FEP Buffer is the fragmentation and end repair reaction buffer. FEP Enzyme Mix is a mixture of enzyme related to fragmentation and end repair.
* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index, the primer sequence is as follows.
Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.
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Illumina High Throughput Sequencing Platform NGS Library Construction Fast DNA Plus Prep Kit K004-A K004-B Images
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