RT-PCR Mix for qPCR
For research use only
GDSBio RT-PCR Mix for qPCR premixed reverse transcription reaction kit R1031
Cat. No.: R1031 Size: 100 rxns (20 µl reaction system)
Components
| Component | R1031 (100 rxns) |
| Enzyme Mix | 100 μl |
| 5X RT Buffer | 400 μl |
| Control Mix | 20 μl |
| RNase-free ddH2O | 1 ml × 2 |
Storage
All components should be stored at -20°C, and avoid repeated freezing and thawing.
Description
RT-PCR Mix for qPCR is a premixed reverse transcription reaction (RT-PCR) kit, easy to operate, can quickly and efficiently complete the reverse transcription reaction. Enzyme Mix contains highly efficient reverse transcriptase and heat labile DNase, which can complete genomic DNA clearance and RT-PCR of RNA in one step. RT Buffer contains other components required for RT-PCR, without the need for multiple addition of components step by step, thus reducing the risk of sample contamination. At the same time, the kit is equipped with Control Mix for a negative control reaction to test for genomic DNA residue in the RNA template. The reverse transcription products of this kit have good compatibility for real-time PCR (qPCR) reaction by dye method and probe method, and can be used for accurate gene expression analysis.
Application
First strand cDNA synthesis.
Important notes
1. This product contains heat labile DNase, so please use each component on ice.
2. To ensure uniform composition, please centrifuge briefly of Enzyme Mix, 5X RT Buffer and Control Mix, then use pipette to blow and mix well before use.
3. During the operation, care should be taken to prevent RNA degradation due to the introduction of RNase. RNA is best stored at -70°C, and avoid repeated freeze-thaw.
4. cDNA products should be stored at -20°C or below, and repeated freeze-thaw should be avoided.
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.
